Morning urine: The first urine excreted in the morning after waking up. Because morning urine is more concentrated, it is conducive to the detection of chemical components, and can also avoid the influence of diet, medication and exercise on the composition of urine Random urine: urine retained at any time Middle urine: used for urine bacterial culture 24-hour urine: generally 6:00 in the morning after the first discharge of urine, that is, to start to retain urine until the next morning 6:00 discharge of all urine, during which attention is paid to the preservation of antiseptic. Sedimentation: 2-3 drops of glutaraldehyde + morning urine 50ml Protein profile, free chemistry, NAG enzyme, RBP: 100ml of morning urine is retained, that is, about half a cup of urine cup Percolation: after dinner on the same day, to 18:00 timing to the next morning within 6:00 no food, no water, urination is not limited, in the next morning at 6:00 punctual urination, emptying the bladder, and then continue to fast from food and water for 1 hour, to 7:00 to retain 10ml of urine Acidification: paraffin oil + 50ml of morning urine The test is usually completed within 30 minutes and should not exceed 2 hours at the longest. Adult female patients should avoid retaining urine specimens during menstruation.