Bone marrow aspiration is an important indicator for confirming the diagnosis of leukemia and is usually not misdiagnosed. Bone marrow aspiration is to extract the patient’s bone marrow fluid for blood system related examination, which can be used for cell morphology examination, hematopoietic stem cell culture, cytogenetic analysis, pathogen biology examination, etc. It has a very important role for bone marrow diseases or blood system diseases, and is commonly used for the diagnosis of leukemia, myeloma and other diseases. Bone marrow aspiration is a common test to confirm the diagnosis of leukemia and is usually not misdiagnosed. For uncertain results, multiple retests can be performed for verification and confirmation. It is also recommended to go to a regular hospital to avoid unsuccessful bone marrow aspiration, which may affect the test results. After bone marrow aspiration, it is necessary to pay attention to rest, strengthen nutrition appropriately, and maintain a good psychological state, which is conducive to the recovery of the disease.