The second and fifth positive items in the hepatitis B two-pair test refer to positive hepatitis B surface antibody and hepatitis B core antibody. If only these two items are positive and the other three items are negative, it indicates that the person has been infected with the hepatitis B virus in the past, but the body’s immune system has since cleared the virus from the bloodstream, which is a self-healing phenomenon after infection. A positive hepatitis B surface antibody indicates that the body has specific immunity against the hepatitis B virus, while a positive hepatitis B core antibody is a marker of having been infected with the hepatitis B virus, but does not mean that the person is now infected. In this case, it means that the person has immunity to the hepatitis B virus, which has a similar effect to the vaccination. However, in this case, the person may still have covalent closed-loop DNA, or cccDNA, of the hepatitis B virus in the liver cells.