The diagnosis of bone tumor is a combination of clinical manifestation, imaging and pathology, and the diagnosis of bone tumor cannot be made by laboratory tests alone. However, laboratory tests still have their own “existence” value: 1. Some patients with osteosarcoma have elevated alkaline phosphatase (AKP), and alkaline phosphatase (AKP) and lactate dehydrogenase (LDH) are important for assessing the prognosis of patients, through detecting AKP level, the recurrence of osteosarcoma and bone metastasis can be detected early. 2, malignant bone tumors often have anemia and decreased hematocrit when they are in advanced stage of wasting manifestation; 3, multiple myeloma often has fast hematocrit, high globulin, A/G inversion, and increased blood calcium may be the manifestation of bone metastatic cancer; 4, PSA is an indicator of bone metastasis of prostate cancer. Although the above laboratory tests cannot diagnose bone tumors, they still have an important role in the diagnosis and treatment of bone tumors.