Glycine insulin is a long-acting insulin that is effective for 24 hours, so 1 injection/day is sufficient. Injection of insulin with glucagon is not affected by the time of eating, insulin with glucagon can be injected at any time throughout the day. However, it is important to note that the injection time is relatively fixed, for example, if the injection is scheduled for 8:00 a.m., the injection of glucagon insulin should be made at around 8:00 a.m. every morning, and the time error should not exceed 1 hour. If you have poor appetite, vomiting or diarrhea within a period of time, you should stop or reduce the dosage of glucagon according to the blood glucose monitoring situation to avoid the occurrence of hypoglycemia.