A fetoprotein of 400ug/L at 26 weeks for twins is not in the normal range and requires further additional testing programs. In a normal pregnancy, whether twins or singletons, the alpha-fetoprotein will not exceed more than 400ug/L. Twins 26 weeks with a fetoprotein of 400 or less and a MOM of less than 2.5 are unlikely to have malformations. Twin fetuses 26 weeks methotrexate 400ug/L need to do further amniocentesis test, or non-invasive DNA test, to rule out the possibility of fetal malformation, and also need to do further liver examination, such as liver CT, MRI, etc.. Twin fetuses 26 weeks methotrexate 400ug/L, need to be timely by the doctor according to the specific circumstances of further examination, to formulate individualized diagnosis and treatment plan, so as not to delay the condition.