Testicular puncture may lead to bleeding in the testicle, causing a testicular hematoma, or the puncture may lead to acute orchitis, testicular enlargement, and pain. Testicular puncture also has the potential to cause disruption of the blood-testis barrier, and the sperm immunosuppressive mechanism can be disrupted, resulting in immune infertility. For testicular puncture, many patients are those with a mass in the testicle, suspected testicular cancer, or those with infertility who have a testicular puncture to confirm the presence of normal sperm production in the testicle. After the testicular puncture, it is recommended that the testicle must be closely monitored for changes, swelling and pain in the testicle, and antimicrobials should be taken after the puncture to prevent infection and hematoma.