The majority of beauty seekers are not unfamiliar with hyaluronidase, which is the so-called “antidote” to micropigmentation, the dissolving enzyme of hyaluronic acid. So, what exactly is hyaluronidase? Why can it dissolve hyaluronic acid? What is the dosage needed to dissolve it? Let’s unravel the mystery one by one. Sources and properties of hyaluronidase Hyaluronidase is made from various sources, including microbial fermentation, animal tissue extraction or genetic recombination. Different hyaluronidases do not have the same amount of hyaluronic acid dissolution, and different types of hyaluronic acid react differently to the same lysis enzyme, so it is crucial to grasp the reasonable dosage. In general, hyaluronidase reacts best with uncross-linked hyaluronic acid, which can be broken down in a few minutes, while it takes longer time for cross-linked hyaluronic acid. In addition, biphasic hyaluronic acid is easier to degrade, while monophasic is relatively difficult because the former is granular and easier to combine with lysozyme, while the latter is agglomerated and less likely to be fully contacted. For hyaluronic acid in the microplastic market, the author has done preliminary experiments and the degradation speed of Rilan is significantly faster. How to use hyaluronidase The common hyaluronidase is usually 1500U, when using it, you can add 10ml of saline to make 150U/ml concentration, in this way, it can usually be dissolved 1:1. The hyaluronidase is injected into the appropriate site at a dose of 1:1, usually 0.05~0.1ml per site, with a slow injection speed and a deeper level rather than a shallow one; for vascular embolism, a higher dose and concentration is required. Usually the injection is effective in 30 min, you can moderate massage to facilitate drug dispersion, and stay for an hour after the injection. Precautions for hyaluronidase use Hyaluronidase is a protein, and all protein-based injections have the potential for allergy, so skin testing can be performed before injection. Hyaluronidase should not be used in the case of local tissue infection, as this will lead to the spread of infection. In addition, botulinum toxin and hyaluronidase should not be injected at the same time, otherwise (here omitted one hundred words, you understand yourself, if there is still unclear, please wildly add Dr. Xin WeChat to ask it).