The procedure of bone puncture: 1, puncture site, often take the anterior superior iliac spine or posterior superior iliac spine as the puncture point, in a few cases choose the sternal stalk, to take the anterior superior iliac spine as an example. 2, puncture point disinfection, wearing gloves, laying sterile cavity towel, with 2% lidocaine local anesthesia. 3, with the left hand thumb, index finger fixed puncture site, the right hand holding the needle vertical bone surface for puncture, when feel contact with the bone, then left and right rotation, slowly drilling When the resistance is felt to disappear and the puncture needle is fixed in the bone, it means that the bone marrow cavity has been entered. 4.Pull out the needle core, attach a 20ml syringe and slowly aspirate the bone marrow fluid, about 0.1-0.2ml. push the bone marrow fluid on a slide and quickly smear 5-6 smears for examination. 5.After aspiration, insert the needle core, pull out the puncture needle, cover the puncture point with pressure of gauze and fix it properly.