Judgment of cortisol level can be initially through clinical symptoms, laboratory tests and other comprehensive judgment. 1. Clinical symptoms: Decreased cortisol, the main manifestations are weight loss, hyperpigmentation, decreased blood pressure, fatigue, weakness, etc. Increased cortisol, the main symptoms are centripetal obesity, full-moon face, hypertension, purpura and ecchymosis, hair growth, acne, etc. 2. Laboratory tests: plasma total cortisol, plasma free cortisol can be measured, usually in the morning and afternoon blood collection and measurement, if necessary, an additional test at midnight; can also determine the 24-hour urine free cortisol content. It is recommended to consult a doctor in time for cortisol level testing, improve the hypothalamus, pituitary gland, adrenal glands and other imaging tests, please follow the doctor’s instructions.