Afternoon urine and morning urine for pregnancy testing depends mainly on the period of pregnancy. If it is at a particularly early stage, before the fertilized egg has been implanted, the afternoon urine is less sensitive than the morning urine. However, after the fertilized egg has become established, the HCG produced by the trophoblast cells has a high specificity, so even the afternoon urine rarely gives a false positive result. Many pharmacies and hospitals nowadays mostly use early pregnancy test strips, which have a high sensitivity to HCG in the urine being tested, so the positive test results are very high. Many of them can be detected even before 20 days. Of course, it is better to use morning urine when testing with the test strips because it is more sensitive, especially for those who want to know earlier if they are pregnant. It is also important to note that the urine test is only the most basic way to confirm pregnancy, but the most important thing is to have an ultrasound to confirm and identify whether it is an intrauterine or ectopic pregnancy. In summary, afternoon urine can detect pregnancy, especially in the early stages of pregnancy. However, in the early stages of pregnancy, afternoon urine is not as sensitive as morning urine, so it is still recommended to use morning urine to test.