Urinary 17-hydroxycorticosteroid (17-OHCS) is a metabolite of adrenocortical hormone, the level of which can reflect the good or bad adrenocortical function, and its measurement and diagnostic value is higher than that of 17-ketosteroid. The assay requires 24-hour urine retention, and requires the addition of 5-10 ml of concentrated hydrochloric acid as a preservative in the retention container in advance, which can be obtained from the relevant laboratory staff. Reference value: Adult male: 27.88±6.6μmol/24h (10.1±2.4mg/24h) Adult female: 23.74±4.47μmol/24h (8.6±1.62mg/24h) Increased urinary 17-hydroxycorticosteroids are commonly found in adrenal cortical tumors, hyperadrenocorticism due to hyperplasia, adrenocortical carcinoma can be significantly In addition, urinary 17-hydroxycorticosteroids may also be increased to varying degrees during heavy hormone therapy, pancreatitis, eclampsia, severe irritation and trauma. The decrease in urinary 17-hydroxycorticosteroids is usually seen in hyperaldosteronism, such as Addison’s disease, anterior pituitary hypofunction, and after adrenalectomy.