Many friends in the hospital after the physical examination, looking at the “+” “-” sign on the laboratory test, confused, the brain keeps flashing “?????” That’s why it’s important to know about hepatitis labs. At present, the most commonly used serological markers for checking HBV infection are HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc, which are referred to as “Hepatitis B two and a half”, and its role is mainly threefold: 1) to determine the presence or absence of HBV infection; 2) to determine the contagiousness of HBV infected people; 3) to serve as a basis for chronic hepatitis B infection. 3) as one of the bases for judging the antiviral treatment and efficacy of chronic hepatitis B patients. So, what are the different clinical significance of different results in these five indicators? Let’s take a look. 1.HBsAg(+),Anti-HBs(-),HBeAg(+),Anti-HBe(-)andAnti-HBc(+):which is commonly known as “triple positive”, indicating that there are HBV infections with active viral replication, and the infectiousness is strong; 2.HBsAg(+),Anti-HBs(-),HBeAg(-),Anti-HBe(-) HBsAg(+),anti-HBs(-),HBeAg(-), anti-HBe(+) and anti-HBc(+): commonly known as “small triple positive”, indicating that there is an HBV infection but viral replication is relatively quiescent, the contagiousness of the relatively weak; 3, HBsAg(+), anti-HBs(-), HBeAg(-), anti-HBe(-) and anti-HBc(+): indicating that there is an HBV infection but viral replication is relatively quiescent, the contagiousness of the HBV infection is relatively weak; 3, HBsAg(+), anti-HBs(-), HBeAg(-), anti-HBe(-) and anti-HBc(+): indicating that there are HBV infection but the virus replication is relatively quiescent, and the infectiousness is relatively weak; 4) HBsAg(-),anti-HBs(+),HBeAg(-),anti-HBe(-) and anti-HBc(+): it indicates that there is a previous HBV infection but protective antibodies have been produced, which is not contagious, and there is no need for hepatitis B vaccine; 5) HBsAg(-), anti-HBs(-), HBeAg( -HBsAg(-),anti-HBs(-),HBeAg(-),anti-HBe(-) and anti-HBc(-): indicating that protective antibodies have been produced after the injection of hepatitis B vaccine, and if hepatitis B vaccine has not been injected, then it indicates that the person has been infected with HBV and has now produced protective antibodies, which are not infectious and do not need to be injected again. It is infectious and does not need to be injected with Hepatitis B vaccine. Here, it should be pointed out that the test results of “Hepatitis B two halves” only reflect the HBV infection and replication and do not fully reflect the severity of the disease, such as “triple positive” disease is not necessarily serious, and “triple positive” disease is not always light. For example, the condition of “major triple positive” is not necessarily serious, while the condition of “minor triple positive” is not always light. To determine the severity of hepatitis B condition, in addition to checking “hepatitis B two halves”, it should also be combined with liver function test, liver fibrosis index and ultrasound, CT performance in order to accurately determine the current condition. In addition, to more accurately determine the replication status of HBV and the contagiousness of the patient, qualitative or quantitative HBV DNA test should also be done.