The normal value for Toxoplasma gondii antibodies is negative if enzyme-linked immunosorbent assay is used. If indirect immunofluorescence therapy is used, the normal value is less than 1:10. Toxoplasma antibodies are clinically significant, and a positive IgG and IgM antibody or IgG antibody titer of ≥1:512 usually indicates recent Toxoplasma infection. If both IgG and IgM antibodies to Toxoplasma are negative, this usually indicates no recent Toxoplasma infection. However, when testing for Toxoplasma gondii antibodies, it is important to note that you should not eat too much fatty or high protein food the day before the blood test, and you should not drink alcohol. The alcohol content in the blood will affect the test results. The test requires a fasting period of 12 hours or more, otherwise the test results will also be affected.