The method of semen retrieval for male semen test is usually through masturbation, which is the most common method of semen retrieval in medicine at present. In general, most hospitals are limited by environmental conditions, and when a male semen test is needed, it is recommended that the patient be allowed to go to the restroom and remove the semen through masturbation. If some large hospitals, or scientific research institutions, are equipped with professional semen extraction room, as well as some sexually stimulating materials, can help patients to get semen through masturbation, so that the patient’s psychological feeling is better, the effect is also better. At present, some hospitals can also provide sterile condoms, which can be used to retrieve semen through sexual intercourse, but it is generally not preferred. The process of sperm retrieval also requires its own physical preparation, avoid excessive sex before sperm retrieval, quit smoking and drinking to be able to ensure the quality of the sperm obtained.