Liver test five refers to the examination of hepatitis B virus five, the examination of negative alanine aminotransferase level increases, consider that there are physiological reasons, there are also pathological reasons. 1. Physiological causes: physiological causes, first of all, may be related to the patient’s emotional anxiety and tension, lack of sleep, staying up late, eating excessively oily food, after avoiding the above triggers, the transaminase level can return to normal. 2. Pathological causes: Pathological causes are more numerous, and it is necessary to exclude the existence of chronic hepatitis C, hepatitis A, hepatitis E, alcoholic hepatitis, autoimmune hepatitis, fatty liver, etc. It is recommended that the patient’s clinical symptoms, relevant ultrasonography, alpha-fetoprotein, and liver stiffness should be combined to assess whether there are the above mentioned pathological problems. It is recommended to consult the gastroenterology department of a regular hospital if the aminotransferase level is found to be elevated, so as not to be negligent in order to avoid delays.