Severe β-degenerate anemia is a pure heterozygote for β0 or β+-degenerate anemia or a double heterozygote for β0 and β+-degenerate anemia, in which β-chain production is completely or almost completely inhibited so that the synthesis of β-chain-containing HbA decreases or disappears and the excess α-chain binds to the γ-chain to become HbF (a2γ2), which results in a significant increase in HbF. Then how to diagnose the shortened life span of red blood cells? According to the clinical characteristics and laboratory tests, combined with a positive family history, the diagnosis can generally be made. Genetic diagnosis can be made when available. Application of radionuclides in vivo or in vitro labeling of erythrocytes, and observe the rate of disappearance of radioactivity in these labeled erythrocytes, so as to obtain the percentage of erythrocyte deaths per day, or erythrocyte renewal of the check points, then 100% of the time required to update the erythrocyte lifespan, and in addition to the use of radionuclides to participate in the generation of erythrocytes, the determination of radioactivity in the disappearance of the time of erythrocyte life. The life span of erythrocytes can also be determined by using radionuclides to participate in erythropoiesis and determining the time of disappearance of radioactivity in erythrocytes. Currently commonly used tracers for 51 sodium chromate and fluorine 32 diisopropyl phosphate: the use of radioactive iron to determine the erythrocyte life expectancy, normal human erythrocyte life expectancy of 100 ~ 130 days, an average of 125 days or so, in addition to the application of 51 chromium labeled erythrocytes in the circulating blood to reduce the 50%, that is, the T50 half-life as a clinical indicator, the method is simpler, the normal person for 25 ~ 40 days, 20 days or less for the shortening of. Below 17 days is a significant shortening, hemolytic anemia patients red blood cell life span is significantly shortened, such as sickle cell anemia shortened to 5~15 days, paroxysmal sleep hemoglobinuria (PNH) shortened to about 10 days. This test is an important method for studying and diagnosing hemolytic anemia. By analyzing the cause of the shortened red blood cell lifespan, it can be confirmed whether the shortened red blood cell lifespan is due to defects in the red blood cells themselves or whether the cell lifespan is shortened due to certain external factors in the patient’s body, while the patient’s own red blood cells are normal. Erythrocytosis erythrocyte life span extension. 1, iron deficiency anemia: light thalassemia clinical manifestations and morphological changes in red blood cells and iron deficiency anemia have similarities, so it is easy to be misdiagnosed. However, iron deficiency anemia often has iron deficiency triggers, serum ferritin content is reduced, the reduction of extra-bone marrow iron granulocyte erythrocytes, erythrocyte free prochlorophyllin is elevated, and iron treatment is effective, etc. It can be differentiated. 2.Infectious hepatitis or cirrhosis: Because HbH disease anemia is mild, accompanied by hepatosplenomegaly, xanthogranuloma, a few cases can also have liver function damage, so it is easy to be misdiagnosed as xanthogranuloma type hepatitis or cirrhosis. However, it can be differentiated by history inquiry, family survey and observation of red blood cell morphology and hemoglobin electrophoresis.