The serological markers that appear in hepatitis B virus infection are hepatitis B surface antigen, hepatitis B surface antibody, hepatitis B e antigen, hepatitis B e antibody, hepatitis B core antigen, and hepatitis B core antibody, as follows: 1. Hepatitis B surface antigen: Hepatitis B surface antigen itself is only antigenic, not infectious, and is the shell of the hepatitis B virus. 2. Hepatitis B surface antibodies are protective antibodies that are produced because of the immune state of the body stimulated by infection with the hepatitis B virus.3. Hepatitis B e antigen: Hepatitis B e antigen indicates that the virus is actively replicating and is highly infectious.4. Hepatitis B e antibody: Hepatitis B e antibody indicates that the virus replication has been in a quiescent state and the infectiousness has decreased.5. Hepatitis B core antibody: generally indicates previous infection with hepatitis B virus. The above items are included in the hepatitis B two-to-one test, the general initial screening, the hepatitis B two-to-one test can first clarify whether the infection of hepatitis B virus.