Cat Scratch Disease is an infectious disease. Clinical diagnosis of Cat Scratch Disease requires tests such as lymph node biopsy and Warthin-Starry silver staining. 1. Lymph node biopsy: the biopsy shows characteristic granulomatous lesions, and the characteristic manifestation in the lymph node is “astrocytic abscess” granuloma, which is characterized by neutrophils in the center, surrounded by fenestrated epithelioid cells, multinucleated giant cells, lymphocytes and fibroblasts, etc.; there may be histiocytic hyperplasia around the granuloma, and enlargement of the center of lymphoid follicular growth. The granuloma may be surrounded by histiocytosis and enlarged lymphoid follicular center. 2. Warthin-Starry silver staining method: the pathogen of cat scratch disease is B. henselae, which can be observed by Warthin-Starry silver staining. B. henselae is weakly Gram-negative, strongly silversmithic staining, polymorphic, round, oval, short rod-shaped, rod-shaped clustered growth, and is located in the necrotic foci and vascular wall. The most reliable and effective way to confirm the diagnosis of cat scratch disease is pathologic biopsy combined with the patient’s history of canine and feline exposure or scratch-and-bite injury. Therefore, patients with a history of cat scratch disease and clinical manifestations of enlarged superficial lymph nodes accompanied by painful masses should seek medical attention, complete the relevant examinations, and follow the doctor’s instructions for early treatment.