The line on the skin where the scrotum holds the testes is called the midline of the scrotum, which extends toward the posterior wall as the mediastinum of the scrotum. The mediolateral scrotum separates the two sides of the scrotum into two separate spaces to accommodate the left and right testicles, so that the testicles on each side do not become entangled and do not interfere with each other. The midline of the scrotum extending toward the body of the penis is called the midline of the penis and is directly fused with the tether of the foreskin, which is the location where the midline of the left and right scrotum is judged and has a positioning effect during surgery. If unilateral orchiectomy or epididymal resection is performed, another incision is required on one side of the midline of the scrotum, otherwise there is a risk of completely cutting both sides of the scrotum and causing unnecessary exposure and injury. Some patients may have a deviation of the midline of the scrotum or a deviation of the foreskin tether during development, but as long as there is no local pain or erectile dysfunction, no special treatment is needed.