The antagonist protocol is the addition of an antagonist to the IVF transfer process to counteract the premature luteinizing hormone peak. Since the antagonist protocol may result in an unstable endometrial state, transferring fresh embryos is not recommended. Antagonists can be used to counteract the premature luteinizing hormone peak in the body during IVF transfer in order to prevent premature follicular maturation and ovulation. However, in addition to inhibiting follicular ovulation, antagonists may also affect the state of the endometrium and even embryo implantation, so it is not recommended to transfer fresh embryos, and frozen embryos can be transferred. After using the antagonist regimen, the dose of progesterone needs to be supplemented to prevent the success rate of IVF from being affected by low progesterone levels. After using the antagonistic protocol, it is also necessary to monitor the changes in estrogen levels and the growth and development of follicles in the woman’s body.