The best time for the six hormone test is the 2nd-3rd day of menstruation to understand ovarian function in the basal state, but it can be done at any time for amenorrheic patients. The six sex hormones include luteinizing hormone, follicle stimulating hormone, progesterone, androgen, and prolactin. On the 2nd-3rd day of the menstrual cycle, the above hormone levels are the basal status of the patient, which can clarify the endocrine function of the patient and help in the diagnosis and treatment of the disease. Before the blood test is drawn, patients are advised to maintain a fasting state. Patients with low blood sugar can consume a small amount of food, but should eat less oily, spicy and other stimulating foods, while sitting still for half an hour to avoid exercise or mood swings affecting the hormone test results in the body. Patients who undergo the six hormone tests should clearly inform the doctor of their menstrual cycle during the visit and follow the doctor’s instructions before the test to avoid affecting the accuracy of the test results and the diagnosis and treatment of the disease.