Osteosarcoma is treated with 3~5 times of preoperative chemotherapy and usually a minimum of 6 times of postoperative chemotherapy. Preoperative chemotherapy of 3~5 times for osteosarcoma can make the tumor shrink, which is conducive to resection. After surgery for osteosarcoma, generally about 6 times of chemotherapy is needed for a course of treatment, and the number of chemotherapy is also needed to be increased according to the difference in the degree of the lesion or whether there is metastasis or not, and the number of times of chemotherapy is also needed to be increased if the surgery does not completely remove the tumor. During the period of chemotherapy, we should review the film regularly, and also review the blood routine to see whether there is any decrease of white blood cells, if there is any decrease of white blood cells, we need to apply chemotherapy with drugs to raise white blood cells, which has a great impact on the body, so we need to strengthen the nutrition. Therefore, how many times of chemotherapy for osteosarcoma should be decided according to the severity of the lesion, and a reasonable chemotherapy program should be formulated, and also regular follow-up to observe the effect of chemotherapy, which should be carried out in accordance with the doctor’s instructions.