First, vaccine factors It was found that the titer of antibody produced by vaccination according to 0, 1 and 6 months immunization program is related to the dose and type of vaccine injected. In addition, there is also a relationship with the preservation of the vaccine. The optimal storage and transportation temperature of the vaccine is 2 degrees Celsius to 10 degrees Celsius. In general, hepatitis B vaccine has good thermal stability, and once frozen, the adjuvant colloid is completely destroyed and the vaccine becomes ineffective. Some people bring home three doses of immunization program vaccine at one time for convenience, but the storage method is not correct, resulting in vaccine failure. Second, the organism factor Research shows that, excluding the factor of detection reagents, more than half of the people with no or weak immune response are themselves hepatitis B virus infected. Generally, the physical examination only checks the hepatitis B surface antigen (HBsAg), and if it is negative, the vaccine is injected. However, studies have shown that 5% to 10% of hepatitis B patients are HBsAg-negative, and these patients do not respond to the hepatitis B vaccine. If the hepatitis B half test indicates anti-HBc positivity, it indicates previous hepatitis B virus infection and it is necessary to review the test with sensitive reagents and to quantify HBV DNA. Some even require a liver tissue biopsy to determine the presence or absence of current hepatitis B virus infection. The immune response to HBsAg in humans is also highly dependent on age and weight. Whether antibodies are produced after vaccination is closely related to the body’s own immune function, so having an immunodeficiency disease (e.g., AIDS), conditions that lead to low immune function (e.g., malignancy, diabetes, kidney dialysis, etc.), and long-term use of immunosuppressive drugs can reduce the body’s antibody response to vaccination. Studies have also shown that people containing certain genes have a poorer immune response to the hepatitis B vaccine. In addition, bad habits can also affect the immune effect, for example, those who smoke more than 5 cigarettes a day have a lower anti-HBs response rate after vaccination than non-smokers, and the same is true for alcoholics. Third, vaccination factors Experiments have shown that the vaccination site is optimal for the deltoid muscle of the upper arm, while other parts of the arm are less effective because, except for the deltoid muscle of the upper arm, other parts are thicker in fat, and the vaccine generally enters the blood circulation only slowly in the fat layer after vaccination, thus affecting the stimulation of immune cells in the body by the vaccine. Within a certain range, anti-HBs titers increase with the number of vaccinations. It has been proved by a large number of population experiments that the currently used immunization program of 0, 1 and 6 months is the most effective, and the World Health Organization (WHO) recommended program of 0, 1 and 2 months is also effective, but it is better to give a booster shot in the 12th month.