What is the difference between Hepatitis B vaccine booster and regular? Hepatitis B vaccine is given once at month 0, once at month 1 and once at month 6, for a total of 3 injections. The first injection is called the regular vaccination, and the injections after 1 month and 6 months are called booster injections. This is because some people may not produce Hepatitis B antibodies after one vaccination, which means that another vaccination after 1 month can often promote the production of Hepatitis B antibodies. If another booster shot is given at 6 months, it can raise the titer even higher in more people or in those who originally had antibodies. Often the number of people who produce antibodies with 3 vaccinations is 96%. Basically, after 3 doses of the hepatitis B vaccine, more than 60% of people produce E antibodies.