Escherichia coli, also known as E. coli, is positive or negative when stained by Gram stain. Escherichia coli belongs to the conditionally pathogenic bacteria, usually its will be through the Gram stain, through the microscope to observe the morphology of Escherichia coli, the number, movement, arrangement, mostly red or pink rod-like structure. Normally exists in the intestinal tract, when the patient’s resistance is poor or displaced to other organs, it will lead to Escherichia coli infection, positive Gram stain. Patients must pay attention to dietary habits in daily life to reduce gastrointestinal tract stimulation and avoid proliferation of Escherichia coli in the intestinal tract. Pay attention to hygiene, wash hands diligently before meals and after bowel movements to reduce Escherichia coli translocation infection and reduce the appearance of Escherichia coli Gram stain positive.