Seminal plasma biochemistry is an important indicator for assessing male fertility. Male semen is mainly composed of spermatozoa and seminal plasma, and there are many important biochemical substances in seminal plasma, which are secreted by the accessory gonads or the reproductive tract, reflecting the function of the accessory gonads to a certain extent, and playing a very important role in maintaining the normal function of spermatozoa and semen, which is of great value in the diagnosis of obstructive azoospermia and non-obstructive azoospermia. The biochemical indicators of seminal plasma commonly used in clinical practice are seminal plasma fructose, α-glucosidase, seminal plasma acid phosphatase, seminal plasma citric acid, seminal plasma elastase and so on.