The N protein is the nucleocapsid protein, and the nucleocapsid protein test is commonly used in the detection of novel coronaviruses with high sensitivity and specificity. Novel coronavirus is mainly composed of four structural proteins, including nucleocapsid protein, spiny protein, membrane protein and envelope protein, and nucleocapsid protein has a higher content among all the above mentioned proteins, which is a highly immunogenic protein, and can be involved in the regulation of cellular signaling pathways and gene replication, etc. The N protein is used to detect the infection of novel coronavirus. It can be involved in cell signaling pathway regulation and gene replication, etc. The sensitivity and specificity of the core coat protein in detecting the infection of novel coronavirus is high. In addition, nucleocapsid proteins generally do not undergo mutation and are less affected by the new coronavirus vaccine, whereas spiny proteins do not persist for a long period of time after vaccination and have a lower positivity rate. In daily life, if there is a recent history of traveling to high-risk areas, or those who have had close contact with infected people, it is necessary to carry out nucleic acid testing in a timely manner. It is recommended to take good personal precautions against the disease in daily life, and it is advisable to wear a good mask when going out. When symptoms such as low-grade fever, fatigue and other related discomforts occur, timely medical attention is also needed.