The role of night injection of Aizawl is mainly to promote better maturation of follicles, it is recommended to be injected around 36 hours after ovulation, in order to facilitate egg retrieval in the morning at the hospital before the injection of Aizawl, Aizawl injection should be done according to the time of follicle development, which can be specific to the minute, injecting too early or too late can have an impact on egg retrieval. It is important to follow the doctor’s instructions when performing nocturnal Aizawl injections and to have the mature eggs removed at the hospital in time after 36 hours of injection. To ensure the quality of eggs, it is recommended to get enough sleep and pay attention to the intake of nutrients, especially high quality protein, and avoid staying up late and being overly tired to create good conditions for the development of quality eggs.