Hepatitis B patients with negative virus quantification indicates that the rate of virus replication in the body is very low, and the infectiousness is relatively low. For hepatitis B virus patients or carriers, when the quantitative examination of hepatitis B virus DNA is negative, it means that the amount of hepatitis B virus in the body is low, and its contagiousness is relatively low. Clinically, the transmission of hepatitis B virus is mainly through blood transmission, sexual contact transmission, and vertical transmission from mother to child, and the hepatitis B two-half-pair test is usually chosen to screen whether the hepatitis B virus is infected or not. If the examiner is negative for both hepatitis B surface antigen and hepatitis B surface antibody, he/she can be injected with hepatitis B vaccine under the instruction of physician to increase resistance. If the hepatitis B surface antigen is positive but the hepatitis B DNA quantification is negative, he/she can choose to review the DNA quantification and liver function tests regularly.