Chromosome, reproductive immunity, hysteroscopy and other tests can be done after the failure of IVF to clarify the cause of failure. 1. Chromosome examination: Chromosome examination is needed for both husband and wife, which not only helps to diagnose whether both husband and wife have chromosome abnormality, but also determines whether the cause of embryo transfer failure is related to chromosome abnormality. 2. Reproductive immunity examination: reproductive immunity examination is for patients with repeated miscarriages and repeated failure of implantation, which can analyze the causes of pregnancy failure from the immunological point of view, find out the causes and exclude genetic, infectious and other factors, and then improve the success rate of pregnancy. Note that when checking reproductive immunity, it is important to avoid the menstrual period, and also fasting. 3. Hysteroscopy: Hysteroscopy is a new type of gynecological diagnostic and treatment technology used for the examination and treatment of the uterine cavity, which can visually and accurately check gynecological bleeding disorders and intrauterine lesions, and find out the reasons for the failure of in vitro fertilization. It is recommended to actively cooperate with the doctor for diagnosis after the failure of IVF transplantation, and also pay attention to maintain a good state of mind on a daily basis, so as not to cause adverse effects on conception.