Immunoblotting tests are relatively accurate in detecting human immunodeficiency virus (HIV), but are not 100% accurate. The immunoblot test is a combination of immunochemical analysis and high-resolution gel electrophoresis. Clinically, the immunoblotting test can be used to detect protein characteristics, distribution, expression, etc., with the advantages of strong specificity and high sensitivity. Immunoblotting test can also be used to detect human immunodeficiency virus, to determine the presence of viral antibodies in the patient’s body, and can also assist in determining whether the patient is suffering from AIDS as a form of examination. Immunoblotting is currently used to confirm HIV antibodies with high accuracy, but the sensitivity and specificity are not 100%, or the results may be in doubt. In most cases, a combination of HIV RNA and HIV antibody testing is required. Regarding the accuracy of human immunodeficiency virus detected by immunoblotting test, it is recommended to consult a professional doctor. In daily life, pay attention to personal hygiene and avoid having unclean sex.