If testicular pain occurs when holding urine, it is recommended to go to the hospital for further examination to clarify the specific cause. 1. There is a urethral infection. In the state of holding urine, there is a high pressure in the urethra, and it is possible for infected urine to enter the seminal mons retrograde and cause infection of the epididymis and testicles. In this case, pain in the testicles and epididymis may occur. When touched with the hand, the testicles and epididymis may appear to increase in size, accompanied by a significant pain. It is recommended that ultrasound and blood tests be performed to confirm the diagnosis, and if the presence of urethral and testicular infections has been confirmed, active anti-infection treatment is required. 2. Prostatic hyperplasia in men can lead to increased resistance in the urethra, which can cause poor urination. The patient always has a feeling of holding urine, while there is a state of high pressure in the urethra, and then there is a possibility of infection of the testicles or epididymis. If the symptoms are not significantly relieved by treating the testicular or epididymal infection alone, surgery or medication for the prostate is needed at the same time to relieve urethral obstruction and improve the symptoms of holding urine before the testicular pain can be relieved.