The clinical significance of the sex hormone six is to understand female endocrine function and diagnose diseases related to endocrine disorders by measuring sex hormone levels. The six sex hormone tests, namely folliculogenic hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P), testosterone (T), and prolactin (PRL), basically satisfy clinicians’ screening for endocrine disorders and general understanding of physiological functions. The hormone levels of women can change at different ages and periods of the menstrual cycle. Therefore, when interpreting the test results, attention should be paid to the method of testing, the time of the test, the age of the person being tested, the units of the test results (the units of the test results may be different due to different methods of testing, and the range of reference values may also vary), and so on, and combined with clinical manifestations (other tests should be referred to if necessary) for a comprehensive analysis.1 The main function of FSH is the development and maturation of follicles in the ovaries. The concentration of FSH is 1.5-10 mIU/ml in the preovulatory period, 8-20 mIU/ml in the ovulatory period and 2-10 mIU/ml in the late ovulatory period. An increase is seen in premature ovarian failure, ovarian insensitivity syndrome, primary amenorrhea, etc. 2. Luteinizing hormone (LH) The main function is to promote ovulation and the formation of luteinizing hormone. The concentration of LH ranges from 2-15 mIU/ml in the preovulatory phase, 20-100 mIU/ml in the ovulatory phase, and 4-10 mIU/ml in the late ovulatory phase. 5 mIU/ml or less is a reliable indication of low gonadotropin function, which is seen in Silhan syndrome. LH/FSH>=3 is one of the bases for the diagnosis of polycystic ovary syndrome.3. Prolactin (PRL) The main function is to promote the production of milk and lactation of the mammary glands. In non-lactating period, the normal value of PRL is 102-496 μIU/ml. increase is seen during pregnancy and postpartum lactation and anorexia nervosa.4. Estradiol (E2) The main function is to make the endometrial glands grow into proliferative phase and promote the development of female secondary sexual characteristics. The concentration of E2 ranges from 13.1-141.96 pg/ml during ovulation, 100.82-500 pg/ml during ovulation, and 74.1-216.1 pg/ml during the late ovulation period, and decreases in ovarian hypoplasia, premature ovarian failure, and Sheehan’s syndrome. The blood P concentration is 0-4.8 mnol/L in the pre-ovulatory phase and 7.6-97.6 nmol/L in the post-ovulatory phase. low P value in the post-ovulatory phase is seen in luteal insufficiency and ovulatory uterine dysfunctional bleeding. 6. testosterone (T) The main function is to promote the development of the clitoris, labia and mons pubis, and has an antagonistic effect on androgens and a certain influence on systemic metabolism. The plasma testosterone level in women is 2.5-7.3 ng/ml, and high T value can cause female infertility.