Lung cancer puncture biopsy is generally painless because it is a local anesthesia operation, in which anesthesia is administered first and no local pain is felt before puncturing and inserting the needle. It is usually performed at the back of the rib cage. Before the operation, CT localization is needed to detect the occupying lesion and then select the appropriate level for puncture. After puncture, CT localization should be done again to make sure that the tumor has been reached inside the lung, and tissue testing should be taken with a trocar needle. After lung cancer puncture, the main observation is whether there is bleeding or infection at the puncture site. There is a risk that pneumothorax may be triggered, which requires postoperative symptomatic treatment such as lying down position and oxygen intake. If chest tightness and chest pain are found, bedside radiographs reveal the formation of pneumothorax and drainage tubes need to be placed.