Prothrombin is a substance that precedes prothrombin activation and is a very important coagulation factor in the body, participating in both the endogenous and exogenous coagulation pathways of the body. When prothrombin is activated into thrombin, it has a powerful coagulation function. At the same time, it can activate fibrinogen to become fibrin, thus forming fibrin multimers to perform the function of coagulation. Therefore, when the concentration of prothrombin in the body is reduced, coagulation dysfunction may occur, leading to various bleeding disorders. In this case, plasma transfusion and cold precipitation are usually used to increase the concentration of prothrombin to treat bleeding disorders due to coagulation disorders.