Coffee is not known to affect nucleic acid testing, but try not to drink it half an hour before sampling. Coffee does not usually affect nucleic acid testing and generally has no effect on nucleic acid testing. Coffee does not kill the novel coronavirus nor does it affect the replication of the virus, so it does not affect the accuracy of the nucleic acid test. However, try not to drink half an hour before sampling, so as not to cause nausea and vomiting and other symptoms during the sampling process. Half an hour before nucleic acid sampling, try not to drink or eat, you can use water to rinse your mouth properly. Taking a throat swab or nasopharyngeal swab may induce nausea, vomiting and other symptoms, and smoking, drinking or eating a large amount of food is not recommended before sampling to avoid aggravating nausea, vomiting and other undesirable manifestations.