Patients with insect bite sensation in the urethra or insect crawling sensation in the vagina need to be examined for the following: 1. History and symptoms without manifestations of a specific type of STD. 2.There are abnormal psychological and behavioral manifestations, with certain quality and personality defects. 3.Psychological and behavioral abnormalities affect normal work and life. The duration of the disease is more than three months. 4.Physical examination and laboratory examination, no positive change of venereal disease. Urine pathogen examination: urine pathogen examination is to check the type and number of pathogens present in the urine to determine whether it is a urinary tract infection and the nature of its infection. Although normal urine is sterile, the external urethra hosts normal flora, so the general urine pathogen test has no clinical significance. A urinary tract infection can be diagnosed when the clean middle urine bacteriological count exceeds 100,000 bacteria/ml. STD research laboratory test: The STD research laboratory test is based on the use of cardiolipin, lecithin and cholesterol as antigens, which can be used for quantitative and qualitative tests, and the reagents and control sera have been standardized, and the cost is low. This method is commonly used, simple to perform, and requires a microscope to read the results, with the disadvantage that the sensitivity of stage 1 syphilis is not high. Anti-cardiolipin antibodies, also known as reactin, are measured in serum using cardiolipin as antigen. This test has high sensitivity but low specificity and is prone to biological false positives. In early syphilis patients, after adequate treatment, the reactin can disappear, and in early untreated patients, in some patients, the reactin can also be reduced or disappeared in late stages. It is now generally used as a screening and quantitative test to observe efficacy, recurrence and reinfection. Syphilis spirochetes cannot be cultured in vitro, nor can they be stained by conventional methods. Common laboratory tests include searching for syphilis spirochetes at typical ulcers and detecting the presence of antibodies in the serum. Different laboratory methods should be used for different stages of infection; ulcers do not appear at any stage of the disease, and antibodies usually appear 1 to 4 weeks after ulcer formation.