Rectal puncture biopsy is a puncture biopsy of a lesion via the rectal route, which can be used for the diagnosis of prostate lesions and gynecological lesions. In general, the procedure is painless. In clinical practice, due to the anatomical peculiarities of the rectum, it is adjacent to the prostate gland in men and the vagina in women. For patients who may have these lesions, the rectal area can be used to puncture these tissues and organs, and then a biopsy can be obtained through the puncture needle for pathologic testing to clarify the final diagnosis. Since the rectum and other organs are innervated by visceral nerves, they do not cause pain to the needle. Therefore, during the puncture process, patients usually do not experience pain, and may experience slight discomfort, so patients should not be overly nervous. Due to the high level of medical care nowadays, the puncture is usually performed under the guidance of ultrasound, and the accuracy of puncture detection is improved. After the puncture, the patient should rest appropriately and try not to move around to prevent bleeding from the puncture site. After the diagnosis is clear, the patient should follow the doctor’s instructions to carry out the appropriate treatment.