Testing during the second week of taking the blocking medication may affect the test results, so it is recommended to test two weeks after the end of the course of treatment, and high-risk individuals must be re-tested for antibodies three months after high-risk behavior to confirm the diagnosis. HIV blocking drugs can largely prevent HIV infection after high-risk behavior, the maximum blocking effect can be achieved within 2 hours, the course of treatment is generally 28 days. Antibody testing during the second week of treatment may affect the results. It is recommended to take the test 2 weeks after the end of the 28-day course of treatment, and if it is negative, the infection can basically be ruled out. High-risk individuals should be retested after 3 months to rule out the diagnosis. In conclusion, there is no need to test during the second week of taking HIV blocking medication. The test can be done 2 weeks after the end of the course of treatment. During the course of taking the medication, it is helpful to follow the doctor’s instructions to take the medication at regular intervals in order to maximize the effect of the blockade.