Objective Partial nephrectomy is currently the main surgical procedure for preserving renal units in renal cancer, and it is generally considered to achieve maximum preservation of residual renal function. For endogenous renal tumors, the difficulty of accurate tumor resection is increased by the unclear intraoperative tumor localization. In this paper, we summarize the experience of applying ultrasound localization in partial nephrectomy and discuss its effectiveness and surgical safety. Methods Eleven cases of partial nephrectomy performed between May 2008 and June 2009 in our department were reviewed, 7 male and 4 female, aged 35-62 years old, average 47.5 years old. There were 5 cases on the left side and 6 cases on the right side, all of which were endogenous renal tumors, with tumor sizes ranging from 1.5 to 3 cm, with an average size of 2.5 cm.Surgical procedure: according to the preoperative imaging data, the perinephric fat of the tumor site and the renal blood vessels of the renal hilum were freed; the renal surface was probed with a Toshiba 3.75 MHz pen-type high-frequency probe to clarify the tumor’s location, size, depth, and relationship with the collecting system and the large blood vessels around the tumor and to exclude any satellite foci around the tumor. Exclude the presence of satellite foci around the tumor; according to the projection line of the tumor contour on the renal surface, make a resection marking line with an electrosurgical knife at a distance of 0.5 cm outward; block the renal blood vessels of the renal hilum; complete resection of the renal tumor and the renal parenchyma of the tumor edge of 0.5 cm along the marking line; suture the bleeding point of the trauma with 3-0 absorbable sutures; fill in the trauma with an absorbable haemostatic gauze roll or perirenal fat, and intermittently suture the trauma with 3-0 absorbable sutures. The wound was filled with absorbable hemostatic gauze roll or perirenal fat and fixed with 3-0 absorbable suture intermittently; the renal blood vessels of the renal pedicle were opened. Results Postoperative pathology: 9 cases of renal clear cell carcinoma, grade I-II, and 2 cases of renal vascular smooth muscle lipoma were negative for surgical margins; patients were followed up for 1-13 months after surgery, and there was no obvious change in renal function compared with that of preoperative period, and there was no local recurrence of tumor or distant metastasis in CT or PET examination. Conclusion Partial nephrectomy is an effective treatment for limited renal cell carcinoma, which can safely and effectively preserve renal function, and its surgical safety and long-term tumor-free survival rate are comparable to that of radical surgery for renal cancer. For endogenous renal tumors, performing intraoperative ultrasound localization can accurately and completely resect the tumor, avoid the residue of tumor and satellite foci, and maximally preserve renal units and protect renal function. It can improve the effectiveness and safety of partial nephrectomy for endogenous renal tumors.