The four liver function tests refer to alanine aminotransferase, total bilirubin, direct bilirubin, and indirect bilirubin. The eight liver function tests refer to total protein, globulin, albumin, albumin, alanine aminotransferase, alanine aminotransferase, total bilirubin, direct bilirubin, and cholinesterase. Clinically, the four and eight liver function tests are mainly used to measure the basic functions of the liver, which means that if there is a problem with the liver, the four and eight liver function tests will be abnormal to varying degrees. If the liver develops viral hepatitis, cirrhosis, or biliary tract tumors, cholecystitis, gallbladder stones, and biliary tract obstruction, alanine aminotransferase, glutamine aminotransferase, total bilirubin, direct bilirubin, and indirect bilirubin will all be abnormal. If a patient develops jaundice, the patient’s total bilirubin and direct and indirect bilirubin will also show different degrees of manifestation, and these three can determine the type of jaundice that the patient is suffering from. Clinically, total protein, globulin, and albumin are used to determine the nutritional status of the patient, while cholinesterase reflects a condition of hepatic metabolism.