Three-dimensional ultrasound examination of the uterus can examine lesions of the uterus and uterine cavity, mainly including malformations of the uterus and lesions of the uterine cavity. Three-dimensional ultrasonography of the uterus can provide three-dimensional localization of endometrial cancer, the extent of lesions involving the myometrium. Three-dimensional ultrasonography of the uterus can provide useful diagnostic information for the staging of endometrial cancer and pregnancy in special areas, in addition to the three-dimensional assessment of polycystic ovarian lesions and tubal effusion. With the increasing incidence of infertility in recent years, three-dimensional uterine ultrasound examination of the endometrium plays a higher role in the evaluation of pregnancy outcomes in embryo in vitro fertilization and transfer. Three-dimensional ultrasound of the uterus can provide three-dimensional diagnostic information about the internal structure and morphology of the lesion as well as its adjacency to surrounding organs.