Ozone has a very strong oxidizing power and a very high reduction potential and can oxidize certain unsaturated chains in the structure of biological tissues. The highest content of proteoglycans is found in the nucleus pulposus (NPS) of the intervertebral disc, which is 40C60% of the dry weight of the NPS. Ozone can specifically oxidize the proteoglycans in the nucleus pulposus of the intervertebral disc, thus decomposing the proteoglycans, leading to a decrease in the osmotic pressure in the disc, and a dehydration and solidification of the nucleus pulposus, thus relieving the pressure of the protruding disc on the nerve root; the proteoglycans themselves are a kind of “foreign substance”, which can stimulate the immune response. Ozone specifically oxidizes proteoglycans, eliminating immunogenic substances; in addition, ozone has an anti-inflammatory and analgesic effect, eliminating chemical and immune inflammation. If the annulus ruptures, ozone can diffuse into the epidural space and the paravertebral space for a broader effect.