Patients with azoospermia who have mature sperm as a result of testicular biopsy can solve their fertility problems through IVF techniques. If there is no mature sperm, fertility can only be achieved by using sperm from a sperm bank. Medication is generally considered ineffective. In small testes, if follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels are high, this often indicates that the lesion is in the testes, in which case, if there is no sperm in the semen, there is usually no sperm in the testes either, and fertility is usually achieved by using sperm from a sperm bank; if follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels are low, this often indicates that the lesion is in the brain, and chorionic gonadotropin and uropoietin can be used. If the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels are low, chorionic gonadotropin and urotropin can be used to achieve fertility in some patients. The general principles of treatment for azoospermia are: the absence of sperm on microscopic examination of semen sediment after two centrifugations can be diagnosed as azoospermia, and azoospermia patients are generally ineffective with drug therapy. Azoospermia patients may have sperm in the testes, in such cases can be removed to do ICSI (single sperm injection, a technique of IVF); testes, if there is no sperm, can only use other people’s sperm to do artificial insemination. It is generally believed that the testicular volume is less than 6 ml and the FSH (follicle stimulating hormone) is 2 times higher than the upper limit of normal, the possibility of finding sperm in the testis is smaller; and vice versa is greater. However, when performing testicular puncture it should be noted that if there is no sperm at one point of puncture, a bilateral multi-point puncture biopsy should be performed. Because testicular sperm production is unbalanced, just as the earth has plains, deserts, and oases in the desert; it is very important for patients to have a testicular biopsy, because there is or is not a direct decision whether the patient takes his own sperm using IVF technology to achieve fertility, or has to use sperm from the sperm bank for artificial insemination, which is, frankly speaking, the question of whether he is the biological father of his offspring The question of whether one is the biological father of the offspring is, frankly, a major issue. Therefore testicular biopsy should be performed at multiple points of puncture if necessary, and strictly speaking a bilateral map testicular puncture biopsy should be performed to obtain a specimen sufficient for laboratory use.