The method of quantitative collection of 24-hour urine protein: First, the items that need to be prepared are plastic bucket capacity, about 2000ml or more, preservatives, such as toluene, and a measuring cylinder. Second, the process of urine collection, according to the doctor’s advice to their own work arrangements, the best choice for urine retention in the morning, such as the same morning at 8 o’clock regardless of whether there is an urge to urinate must be urinated once, and this time the urine will be discarded. However, all urine within 24 hours afterwards should be put into a plastic bucket and the preservative added after the first urine and shaken well. On the second day, at 8:00 a.m., you must urinate once, again regardless of whether you have urinated visibly, and put the urine into a plastic bucket.