Objective To evaluate the efficacy and safety of long-pulsed Nd:YAG laser treatment for glans venous malformation. Methods Ten patients with glans venous malformation were treated with long-pulsed 1064 nm Nd:YAG laser with 7-8 ms double pulse width, 20 ms pulse interval, 120-140 J/cm2 energy, contact cooling, 1 month treatment interval, and 12 months follow-up after the course of treatment to evaluate the lesion clearance rate in five grades and observe complications. Results The maximum number of treatments was 7. At 12 months of follow-up, all patients had complete clearance of the glans venous malformation lesions, and no recurrence was observed. One patient had mild pain during treatment and all had temporary erythema and edema at the treatment site. There was no bleeding, infection, pigmentation changes, scar formation or erectile dysfunction. Conclusion The long-pulse Nd:YAG laser can safely and effectively treat glans venous malformation with few complications, and can be used as the first-line treatment for glans venous malformation.