Normal human urine is generally sterile Urine culture is mostly used to check the pathogenic bacteria of urinary tract infections, such as cystourethritis and pyelonephritis, and can check the sensitivity of pathogenic bacteria to antibiotics, thus helping to select effective antibiotics. Urine culture specimens should be collected to avoid contamination by miscellaneous bacteria. A sterile catheter can be used to obtain urine, and the mid-phase urine is mostly taken clinically. The urethral orifice can be washed with soap and water or warm water, then disinfected with Neosporin, etc., and then the middle urine can be discharged into a sterile container for testing. You can also centrifuge the urine and take the sediment to smear the bacteria. A urine culture with a colony count of 100,000 or more per ml has diagnostic significance. In patients with urinary tract infections such as cystitis and pyelonephritis, Escherichia coli, E. coli, E. paracoccus, Enterococcus faecalis, Streptococcus, Staphylococcus, Aspergillus, etc. can often be detected in the urinary tract. If a urine culture does not detect pathogenic bacteria, the presence of a urinary tract infection cannot be denied. Because there are many factors affecting the culture results, such as the application of antibacterial drugs, diuresis or large amounts of water urine dilution, can make a large number of bacteria in the urine reduced, resulting in a negative culture. Therefore, a comprehensive clinical judgment is needed to treat urinary tract infections by combining morbid characteristic symptoms, physical examination, urinary routine and culture.