Including semen volume, appearance, liquefaction time, pH, sperm count, motility, survival rate and morphology. (1) Volume: Normal male ejaculate volume is ≧2ml at one time, generally 2~6ml, of course, under the premise of abstinence for 3~7 days. Too little semen volume is not conducive to sperm entering the uterus and fallopian tubes through the vagina, affecting conception. Too much semen volume, sperms are diluted, also unfavorable to fertility. (2) Appearance: Normal semen is milky white, grayish-white, homogeneous and semi-fluid. If the semen is light yellow, it is often seen that the time of abstinence is too long; if it is red or coffee-colored, there may be inflammation of the prostate or seminal vesicle glands. (3) Liquefaction time: Liquefaction time refers to the time it takes for normal semen to change from jelly to liquid after ejaculation. The liquefaction time of normal human semen is generally 15-30min, if more than 60min, is still jelly-like, it is a pathological condition, called semen not liquefied. The cause of semen non-liquefaction is not completely clear, it may be due to seminal vesiculitis or prostatitis caused by prostate secretion of fibrinolytic enzyme is insufficient, or trace elements (magnesium, zinc, etc.) lack of, and so on. (4) semen odor: semen has a special fishy smell, this smell is the oxidation of sperm ammonia, sperm ammonia secreted by the prostate gland, if the lack of this smell indicates that there may be inflammation of the prostate gland. (5) pH: the normal value is 7.2～8.0. pH>8.0 is common in acute epididymitis, vesicoureteral adenitis, prostatitis, etc.; pH<7.2 is common in congenital vasovagal blockage, congenital seminal vesicle or epididymal hypoplasia. (6) Sperm count: generally 15×106/ml; less than this value is considered as oligospermia, which is characterized by low spermatozoa and often affects conception. (7) Sperm viability: Sperm viability refers to the motility of sperm, which is categorized into three levels by WHO: PR means spermatozoa are fast and straight forward, moving in a straight line; NP means spermatozoa are not moving forward, moving in place; and IM means spermatozoa are inactive, not moving. Normally, PR spermatozoa are ≥32%, and if PR spermatozoa are <32%, they are considered weak spermatozoa. (8) Sperm survival rate: Sperm survival rate refers to the proportion of surviving spermatozoa to the total number of spermatozoa. The sperm survival rate of a normal person should be ≧58%. (9) Sperm morphology examination: It is mainly to check the malformation of sperms. The proportion of normal sperm with normal morphology should be ≥4% in normal people; when the proportion of malformed sperm is >96%, it can cause fertility disorder and even lead to cessation of embryo development or spontaneous abortion in the female partner.