When you think of ozone, you must think of the ozone layer in the atmosphere, and you must be surprised that ozone can also cure diseases? What is ozone all about? Most of the ozone in nature exists between 14,000 and 50,000 meters from the ground. We know that twenty-one percent of the air is oxygen, and when the oxygen molecules in the atmosphere are exposed to short-wave ultraviolet light from the sun, the oxygen molecules break down into their atomic state. Oxygen atoms are very unstable and can easily react with other substances, such as reacting with hydrogen (H?) to produce water (H?O), and carbon (C) to produce carbon dioxide (C0?); if reacting with oxygen molecules (O?), they form a triple oxygen molecule (O?), or ozone. Ozone is composed of the same oxygen element, with a different molecular structure than oxygen, an isotope, which is colorless but has a pungent odor, so it gets this notorious name. Ozone has a considerable concentration in the atmosphere in the region from 10 km to 50 km altitude above the ground, encircling the outer space of the Earth, called the ozone layer. Because its specific gravity is greater than that of oxygen, ozone will gradually descend to the bottom of the ozone layer, and during the descent, ozone will also rebound as the temperature rises, which means that as ozone sinks, its instability becomes more pronounced. Natural ozone is sufficient to absorb ultraviolet rays below 306.3 μm in sunlight to protect humans, plants and animals on earth from short-wave ultraviolet radiation. Only about 1% of the sun’s ultraviolet rays can reach the ground. The ozone layer actually maintains a dynamic balance of oxygen and ozone conversion to each other and becomes the umbrella for human survival. Ozone has extremely strong oxidizing properties. In forests, mountains and around the coast where atmospheric pollution is lighter, there is more ultraviolet light and there is more abundant ozone, and the right amount of ozone often makes people feel refreshed and invigorated; however, the excessive oxidizing properties also make it lethal. Human knowledge of ozone can be traced back to 1840, when the German chemist Dr. Schanbein, observing water electrolysis and spark discharge, always smelled an unpleasant odor, which in turn proved that the gas was composed of three oxygen atoms with a ring-like structure of ozone molecules, and hence laid the foundation for the artificial production of ozone. So far, the main methods of medical ozone production are silent discharge, nuclear radiation, ionization, plasma jet and ultraviolet irradiation. Ozone has been used as human health care for a long time, such as healing in the seaside, mountains and forests; in 1870 people started to use ozone to purify blood, and in World War I people used ozone to disinfect wounds; then people tried to use ozone to treat infectious and non-infectious diseases, and in the 1970s, Europe and America standardized ozone research and treatment, and found that ozone has certain curative effects on many diseases. In 2000, Guangzhou Southern Hospital took the lead in applying ozone gas soft tissue injection to explore the treatment of disc prolapse and found that ozone could oxidize proteoglycans, causing them to lose their fixed charge density, decrease osmotic pressure and water in the nucleus pulposus; in addition, ozone caused necrosis and decreased function of nucleus pulposus cells and reduced the ability to produce proteoglycans, thus achieving the effect of treating disc prolapse. This method has also been applied to treat prostate hypertrophy, superficial benign and malignant tumors and hemangiomas. Ozone is very soluble in water, and its solubility is 13 times that of oxygen and 25 times that of air at one standard air pressure at room temperature. Foreign scholars dissolved ozone in vitro into their own blood and then gave it back to patients through intravenous infusion for the treatment of hepatitis C and hepatitis B. Chinese scholars also found through clinical trials that the combined response rate of hepatitis B virus DNA, serological markers and blood biochemical indexes was 13.3% and partial response rate was 46.7% after 3 months of treatment with medical ozone alone for chronic hepatitis patients, and virological response (serum HBV DNA decreased by more than 2 log10% after treatment). HBV DNA decreased by more than 2 log10 after treatment) rate of 31.1%, and HBeAg serological conversion rate of 28.6%. Medical ozone combined with adefovir for the treatment of chronic hepatitis B. The results showed that the combined treatment group could improve the seroconversion rate of hepatitis B virus e antigen and antiviral efficacy. The mechanism of medical ozone treatment for chronic liver disease Guangzhou Southern Hospital scholars believe that medical ozone can stimulate the body’s leukocyte proliferation, enhance the phagocytosis of granulocytes, stimulate the formation of monocytes, activate immune active cells, promote the release of cytokines; medical ozone by acting with the body, instantly increase the number of free radicals, induce and activate the body’s antioxidant enzyme system, improve cellular anti-free radical antioxidant effect. Medical ozone can also activate red blood cells, increase the level of adenosine triphosphate (ATP) and 2,3-diphosphoglycerol (2,3-DGP), increase blood oxygen saturation, improve blood circulation, activate cellular metabolism, improve the tissue activity of the body, and make the tissue supply Oxygenation is improved and microcirculation is improved. The increase of drug-resistant pathogens in cirrhotic ascites, especially in spontaneous bacterial peritonitis, has made treatment difficult in recent years and has become an important factor leading to the aggravation of the patient’s condition. Ozone is not resistant to either antibacterial or antiviral agents, and clinical medical researchers believe that medical ozone could be a new treatment option for cirrhosis combined with spontaneous bacterial peritonitis. In 1980, 644 experts from the German medical community retrospectively analyzed 5,579,238 cases of ozone therapy and found that only 40 of them had side effects such as allergic reactions, with an incidence rate of 7 per 100,000. This study suggests that ozone therapy is a safe treatment method. China has also organized a national multi-regional multi-center randomized ozone therapy clinical trial for chronic liver disease with the participation of several tertiary hospitals, including Guangzhou Southern Hospital, Beijing 302 Hospital and Shanghai Public Health Clinical Center, to explore new methods of treating hepatitis with ozone alone or in combination with interferon and nucleoside antiviral drugs, especially for those patients with liver disease who do not want to receive interferon and nucleoside drugs.